Kungani iPolyyacrylamise isetshenziselwa amaprotheni electrophoresis

Endaweni yesayensi yanamuhla, amaprotheni e-electrophoresis ami njengendlela ye-Cornerstone yokuhlaziya nokubonisa amaprotheni. Enhliziyweni yale ndlela amangaPolycrylamide, Ikhompiyutha eguquguqukayo esebenza njengethambo lomgogodla wama-matric we-gel asetshenziswa ezinhlelweni ze-electophoresoresis ze-gel. Izakhiwo ezihlukile zikaPolyRacrylide zenza kube yithuluzi elisemqoka labaphenyi nososayensi abafuna ukwembula ubunzima beprotheni nokusebenzisana kwabo.

I-PolyCrylamide, evame ukubizwa ngokuthi i-Pam, iyi-polymer yokwenziwa eyenziwe ngama-acrymide monomers. Ukusebenza kwayo okuphawuleka okuphawuleka kuthiwa yikhono lawo lokwakha amaketanga amade, okuholela entweni efana ne-gel-efana nokwamukela osayizi abahlukahlukene ama-molecule. Le mpahla yenza ama-polycrylamide abe umuntu ofanelekile ngokwakha ama-matric asetshenziselwa ama-protein electrophoresis.

I-Protein Electrophoresis yindlela ehlukanisa amaprotheni asuselwa ekushajaneni nasekukhulumeni. Ngokubheka isampula le-protein emkhakheni kagesi ngaphakathi kwe-polyracide gel matrix, amaprotheni athutha ngejeli ngamanani ahlukene, okuholela emabhendi ahlukile angahlaziywa futhi ahlukaniswe. Lokhu kuhlukaniswa kunikeza ukuqonda okubalulekile ekuhlanzeni amaprotheni, ukuzimisela kwesisindo kwamangqamuzana, kanye nokuba khona kwama-isoform.

Indima ye-polyyamide ku-protein electrophoresis

Ukukhethwa kwePolyyamide ye-Protein Electrophoresis kusekelwe kumvelo yawo. Ososayensi bangaguqula ukugcwaliseka kwe-matrix ye-gel ukuze kuhlaliswe amaprotheni osayizi abahlukahlukene. Ukugxila okuphezulu kwakha ama-matric aphansi alungele ukuxazulula amaprotheni amancane, ngenkathi ukugxila okuphansi kusetshenziselwa amaprotheni amakhulu. Lokhu kuvumelana nezimo kuqinisekisa ukuthi abacwaningi bangakwazi ukuvumelanisa izivivinyo zabo ukufezekisa ukuhlukaniswa okuphezulu nokuhlaziywa.

Poyacrylamide njengeI-flecculontant

I-PolyRacrylide's Unility inwebeka ngaphezu kwendima yayo ku-gel electrophoresis. Ithola futhi izinhlelo zokusebenza njenge-flocculant ezimbonini ezahlukahlukene, njengokuphathwa kwamanzi kanye nokuphathwa kwamanzi amdaka. Njenge-flocculant, ama-polyyamide ingculaza ehlanganise izinhlayiya ezimisiwe ku-ketshezi, okwenza lula ukususwa kwazo. Lesi sici siqokomisa amakhono ahlukahlukene ahlukahlukene futhi nomthelela obanzi ovela kwisayensi nasembonini.

Intuthuko ku-Electrophoresis esekelwe ePoyacryRalide

Eminyakeni yamuva nje ngibone intuthuko eqhubekayo emasusi ase-Electrophoresis ase-polycrylamide. Ikhasi Lendabuko, ikhasi le-GEL-Page, kanye ne-Electrophoresis enezici ezimbili nje zezinguquko ezimbalwa zendlela ukuzivumelanisa nezimo ze-polylacrylamide zinikeze amandla ukuthuthukiswa kwezindlela ezikhethekile zokuhlaziya izinhlaka zamaprotheni, ukuguqulwa kwangemva kokuhumusha, nokusebenzisana. Lezi zinqubo zibaluleke kakhulu kuma-proteomics ucwaningo kanye nemizamo yokuthola izidakamizwa.

Endaweni yokuhlaziywa kwamaprotheni, ama-polyyamide aqhamuka njengomlingani we-stalwart, enika abacwaningi ukuthi basebenzise izwe eliyinkimbinkimbi lamaprotheni. Indima yayo njengesisekelo se-gel matrices ezinsizeni ze-electrophoresis ayinakweqiwa. Ukusuka ekuvikeleni izindlela zokuhlakulela amanoveli zokwelapha, ama-electoferesis asuselwa ku-polylacrylamide aqhubeka abumba inqubekela phambili yesayensi. Njengoba ubuchwepheshe buthuthuka, lesi simangaliso sokwenziwa cishe sizovela, sithuthukise futhi ukuqonda kwethu amaprotheni kanye neminye imikhakha.