Emkhakheni wesayensi yesimanje, i-protein electrophoresis ime njengendlela yetshe legumbi yokuhlaziya nokuhlukanisa amaprotheni.Enhliziyweni yale ndlela yokusebenza kuleleI-Polyacrylamide, isakhi esisebenza ngezindlela eziningi esisebenza njengomgogodla wamatrices ejeli asetshenziswa ezinhlelweni ze-gel electrophoresis.Izakhiwo ezihlukile zePolyacrylamide ziyenza ibe ithuluzi elibalulekile kubacwaningi nososayensi abafuna ukwembula ubunkimbinkimbi bamaprotheni nokusebenzisana kwawo.
I-Polyacrylamide, evame ukubizwa ngokuthi i-PAM, i-polymer yokwenziwa eyenziwe ngama-acrylamide monomers.Ukuguquguquka kwayo okumangalisayo kubangelwa ikhono layo lokwakha amaketanga amade, okuphumela entweni efana nejeli ekwazi ukumumatha osayizi abahlukahlukene bama-molecule.Lesi sakhiwo senza i-polyacrylamide ibe yikhandidethi elifanele lokudala u-matrics onama-porous asetshenziswa ku-protein electrophoresis.
I-protein electrophoresis iyindlela ehlukanisa amaprotheni ngokusekelwe emalini yawo kanye nosayizi.Ngokufaka isampula yamaprotheni endaweni kagesi ngaphakathi kwe-matrix yejeli ye-polyacrylamide, amaprotheni ahamba ngejeli ngamanani ahlukene, okuholela kumabhendi ahlukile angahlaziywa futhi abalwe.Lokhu kuhlukaniswa kunikeza ukuqonda okubalulekile ekuhlanzekeni kwamaprotheni, ukunqunywa kwesisindo samangqamuzana, kanye nokuba khona kwama-isoforms.
Indima ye-Polyacrylamide ku-Protein Electrophoresis
Ukukhethwa kwe-polyacrylamide ye-protein electrophoresis kusekelwe esimweni sayo esingaguquleka.Ososayensi bangalungisa ukuhlangana kwe-matrix yejeli ukuze kufakwe amaprotheni anosayizi abahlukahlukene.Ukugxila okuphezulu kudala ama-matrics aqinile afanele ukuxazulula amaprotheni amancane, kuyilapho ukugxila okuphansi kusetshenziselwa amaprotheni amakhulu.Lokhu kuzivumelanisa nezimo kuqinisekisa ukuthi abacwaningi bangakwazi ukulungisa izivivinyo zabo ukuze bathole ukuhlukana nokuhlaziya okuphelele.
Polyacrylamide njengobaI-Flocculant
Umsebenzi we-Polyacrylamide udlulela ngale kwendima yawo ku-gel electrophoresis.Iphinde ithole izinhlelo zokusebenza njenge-flocculant ezimbonini ezihlukahlukene, njengokuhlanza amanzi nokuphathwa kwamanzi angcolile.Njenge-flocculant, i-polyacrylamide isiza ekuhlanganiseni izinhlayiya ezimisiwe oketshezini, kube lula ukususwa kwazo.Lesi sici sigqamisa amakhono ahlukahlukene ekhompiyutha kanye nomthelela obanzi kusayensi nezimboni.
Intuthuko ku-Polyacrylamide-Based Electrophoresis
Iminyaka yakamuva ibone ukuthuthuka okuqhubekayo kumasu we-electrophoresis asuselwa ku-polyacrylamide.IKHASI Lomdabu, i-SDS-PAGE, kanye ne-electrophoresis yejeli enezinhlangothi ezimbili kuyizibonelo ezimbalwa zokuthi ukuzivumelanisa nezimo kwe-polyacrylamide kwenze kanjani ukuthuthukiswa kwezindlela ezikhethekile zokuhlaziya izakhiwo zamaprotheni, ukuguqulwa kwangemuva kokuhumusha, nokusebenzisana.Lezi zindlela zibalulekile ocwaningweni lwe-proteomics nasemizameni yokutholwa kwezidakamizwa.
Emkhakheni wokuhlaziya amaprotheni, i-polyacrylamide ivela njengomngane oqinile, okwenza abacwaningi bakwazi ukungena ezweni eliyinkimbinkimbi lamaprotheni.Indima yayo njengesisekelo se-gel matrices ezinhlelweni ze-electrophoresis ayikwazi ukweqiwa.Ukusuka ekuxazululeni izindlela zezifo kuye ekuthuthukiseni izindlela zokwelapha ezintsha, i-electrophoresis esekwe ku-polyacrylamide iyaqhubeka nokubumba inqubekelaphambili yesayensi.Njengoba ubuchwepheshe buthuthuka, lesi simangaliso sokwenziwa cishe sizoshintsha, sithuthukise ukuqonda kwethu amaprotheni nemisebenzi yawo eminingi.
Isikhathi sokuthumela: Aug-21-2023